Luke Gilbert, PhD

A systematic search for RNA structural switches across the human transcriptome.

Nature methods

Khoroshkin M, Asarnow D, Zhou S, Navickas A, Winters A, Goudreau J, Zhou SK, Yu J, Palka C, Fish L, Borah A, Yousefi K, Carpenter C, Ansel KM, Cheng Y, Gilbert LA, Goodarzi H

Genome-wide quantification of RNA flow across subcellular compartments reveals determinants of the mammalian transcript life cycle.

Molecular cell

Ietswaart R, Smalec BM, Xu A, Choquet K, McShane E, Jowhar ZM, Guegler CK, Baxter-Koenigs AR, West ER, Fu BXH, Gilbert L, Floor SN, Churchman LS

Engineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations.

Nature biotechnology

Hsiung CC, Wilson CM, Sambold NA, Dai R, Chen Q, Teyssier N, Misiukiewicz S, Arab A, O'Loughlin T, Cofsky JC, Shi J, Gilbert LA

Improving prime editing with an endogenous small RNA-binding protein.

Nature

Yan J, Oyler-Castrillo P, Ravisankar P, Ward CC, Levesque S, Jing Y, Simpson D, Zhao A, Li H, Yan W, Goudy L, Schmidt R, Solley SC, Gilbert LA, Chan MM, Bauer DE, Marson A, Parsons LR, Adamson B

Higher-order combinatorial chromatin perturbations by engineered CRISPR-Cas12a for functional genomics.

bioRxiv : the preprint server for biology

Hsiung CC, Wilson CM, Sambold NA, Dai R, Chen Q, Misiukiewicz S, Arab A, Teyssier N, O'Loughlin T, Cofsky JC, Shi J, Gilbert LA

Epigenetic reprogramming shapes the cellular landscape of schwannoma.

Nature communications

Liu SJ, Casey-Clyde T, Cho NW, Swinderman J, Pekmezci M, Dougherty MC, Foster K, Chen WC, Villanueva-Meyer JE, Swaney DL, Vasudevan HN, Choudhury A, Pak J, Breshears JD, Lang UE, Eaton CD, Hiam-Galvez KJ, Stevenson E, Chen KH, Lien BV, Wu D, Braunstein SE, Sneed PK, Magill ST, Lim D, McDermott MW, Berger MS, Perry A, Krogan NJ, Hansen MR, Spitzer MH, Gilbert L, Theodosopoulos PV, Raleigh DR

ENPP1 is an innate immune checkpoint of the anticancer cGAMP-STING pathway in breast cancer.

Proceedings of the National Academy of Sciences of the United States of America

Wang S, Böhnert V, Joseph AJ, Sudaryo V, Skariah G, Swinderman JT, Yu FB, Subramanyam V, Wolf DM, Lyu X, Gilbert LA, Van't Veer LJ, Goodarzi H, Li L

Base-editing mutagenesis maps alleles to tune human T cell functions.

Nature

Schmidt R, Ward CC, Dajani R, Armour-Garb Z, Ota M, Allain V, Hernandez R, Layeghi M, Xing G, Goudy L, Dorovskyi D, Wang C, Chen YY, Ye CJ, Shy BR, Gilbert LA, Eyquem J, Pritchard JK, Dodgson SE, Marson A

Mediator Kinase/CDK8 Inhibition As a Strategy to Improve FLT3 Inhibitor Activity in Acute Myeloid Leukemia.

Blood

Timothy T. Ferng, Samantha M. Pintar, Vanessa E. Kennedy, Theodore C. Tarver, Shaheen Kabir, Veronica Steri, Juan Antonio Camara Serrano, Fernando Salangsang, Paul Phojanakong, Juwita Hübner, Carolina E. Morales, Jose M. Rivera, Aaron C. Logan, Benjamin Braun, Elliot Stieglitz, Luke A. Gilbert, Catherine C. Smith

EPCO-12. IN VIVO PERTURB-SEQ DEFINES BIOLOGICAL DRIVERS AND THERAPEUTIC VULNERABILITIES UNDERLYING RADIOTHERAPY RESISTANCE IN GLIOBLASTOMA.

Neuro-Oncology

John Liu, Joanna Pak, Christopher Zou, Sixuan Pan, Emily Payne, Kyla Foster, Katie Lin, Tim Casey-Clyde, Kyounghee Seo, Thomas O'Loughlin, David Wu, Daniel Lim, Harish Vasudevan, Aaron Diaz, Tomoko Ozawa, Mitchel Berger, John de Groot, William Weiss, Luke Gilbert, David Raleigh

EXTH-78. CRISPROFF EPIGENOME EDITING AND FUNCTIONAL GENOMICS DEFINE MEDIATORS OF CHEMOTHERAPY RESISTANCE IN GLIOBLASTOMA.

Neuro-Oncology

Katie Lin, Laine Goudy, Joanna Pak, Kyla Foster, Emily Payne, Tomoko Ozawa, John de Groot, Harish Vasudevan, David Raleigh, Luke Gilbert, Mitchel Berger, John Liu

CRISPR-Based Epigenome Editing and Genome Wide Screening Define Mediators of Chemotherapy Response in Glioblastoma.

International Journal of Radiation Oncology • Biology • Physics

K. Lin, L. Goudy, J. Pak, K. Foster, E. Payne, T. Ozawa, J. de Groot, H. Vasudevan, D. Raleigh, L.A. Gilbert, M.S. Berger, S.J. Liu

Identifying Gene-Treatment Interactions and Targetable Radiation Vulnerabilities in Glioblastoma through Coupling of In Vivo CRISPR Perturbation and Single Cell Transcriptomics.

International Journal of Radiation Oncology • Biology • Physics

S.J. Liu, J. Pak, C. Zou, E. Payne, K. Foster, H. Vasudevan, T. Casey-Clyde, K. Seo, T. O'Loughlin, D. Wu, D. Lim, T. Ozawa, J. de Groot, M.S. Berger, W. Weiss, L.A. Gilbert, D. Raleigh

Environmental challenge rewires functional connections among human genes.

bioRxiv : the preprint server for biology

Herken BW, Wong GT, Norman TM, Gilbert LA

ENPP1 is an innate immune checkpoint of the anticancer cGAMP-STING pathway.

bioRxiv : the preprint server for biology

Wang S, Böhnert V, Joseph AJ, Sudaryo V, Swinderman J, B Yu F, Lyu X, Skariah G, Subramanyam V, Gilbert LA, Goodarzi H, Li L

Integrative identification of non-coding regulatory regions driving metastatic prostate cancer.

bioRxiv : the preprint server for biology

Woo BJ, Moussavi-Baygi R, Karner H, Karimzadeh M, Garcia K, Joshi T, Yin K, Navickas A, Gilbert LA, Wang B, Asgharian H, Feng FY, Goodarzi H

Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors.

eLife

Replogle JM, Bonnar JL, Pogson AN, Liem CR, Maier NK, Ding Y, Russell BJ, Wang X, Leng K, Guna A, Norman TM, Pak RA, Ramos DM, Ward ME, Gilbert LA, Kampmann M, Weissman JS, Jost M

IFITM proteins assist cellular uptake of diverse linked chemotypes.

Science (New York, N.Y.)

Lou K, Wassarman DR, Yang T, Paung Y, Zhang Z, O'Loughlin TA, Moore MK, Egan RK, Greninger P, Benes CH, Seeliger MA, Taunton J, Gilbert LA, Shokat KM

The 5-Hydroxymethylcytosine Landscape of Prostate Cancer.

Cancer research

Sjöström M, Zhao SG, Levy S, Zhang M, Ning Y, Shrestha R, Lundberg A, Herberts C, Foye A, Aggarwal R, Hua JT, Li H, Bergamaschi A, Maurice-Dror C, Maheshwari A, Chen S, Ng SWS, Ye W, Petricca J, Fraser M, Chesner L, Perry MD, Moreno-Rodriguez T, Chen WS, Alumkal JJ, Chou J, Morgans AK, Beer TM, Thomas GV, Gleave M, Lloyd P, Phillips T, McCarthy E, Haffner MC, Zoubeidi A, Annala M, Reiter RE, Rettig MB, Witte ON, Fong L, Bose R, Huang FW, Luo J, Bjartell A, Lang JM, Mahajan NP, Lara PN, Evans CP, Tran PT, Posadas EM, He C, Cui XL, Huang J, Zwart W, Gilbert LA, Maher CA, Boutros PC, Chi KN, Ashworth A, Small EJ, He HH, Wyatt AW, Quigley DA, Feng FY

Abstract B021: Long non-coding RNAs that are required for robust cell growth in ewing Sarcoma.

Clinical Cancer Research

Marcela C. Briones Martin del Campo, Alex Lee, Max Horlbeck, Truc Dinh, Marta Roman-Moreno, Leanne Sayles, Bokyung Seong, Kimberly Stegmaier, Luke Gilbert, E. Alejandro Sweet-Cordero

Highly efficient generation of isogenic pluripotent stem cell models using prime editing.

eLife

Li H, Busquets O, Verma Y, Syed KM, Kutnowski N, Pangilinan GR, Gilbert LA, Bateup HS, Rio DC, Hockemeyer D, Soldner F

A campaign targeting a conserved Hsp70 binding site uncovers how subcellular localization is linked to distinct biological activities.

Cell chemical biology

Shao H, Taguwa S, Gilbert L, Shkedi A, Sannino S, Guerriero CJ, Gale-Day ZJ, Young ZT, Brodsky JL, Weissman J, Gestwicki JE, Frydman J

Applications of CRISPRi and CRISPRa in Drug Discovery.

Luke A. Gilbert

A pan-CRISPR analysis of mammalian cell specificity identifies ultra-compact sgRNA subsets for genome-scale experiments.

Nature communications

Zhao B, Rao Y, Leighow S, O'Brien EP, Gilbert L, Pritchard JR

RNA Polymerase II Is a Therapeutic Target to Overcome FLT3 Inhibitor Resistance Mediated By the Bone Marrow Microenvironment.

Blood

Timothy T. Ferng, Theodore C. Tarver, Shaheen Kabir, Benjamin Braun, Aaron C. Logan, Luke A. Gilbert, Catherine C. Smith

A global cancer data integrator reveals principles of synthetic lethality, sex disparity and immunotherapy.

Genome medicine

Yogodzinski C, Arab A, Pritchard JR, Goodarzi H, Gilbert LA

A new era in functional genomics screens.

Nature reviews. Genetics

Przybyla L, Gilbert LA

An integrated functional and clinical genomics approach reveals genes driving aggressive metastatic prostate cancer.

Nature communications

Das R, Sjöström M, Shrestha R, Yogodzinski C, Egusa EA, Chesner LN, Chen WS, Chou J, Dang DK, Swinderman JT, Ge A, Hua JT, Kabir S, Quigley DA, Small EJ, Ashworth A, Feng FY, Gilbert LA

Revealing molecular pathways for cancer cell fitness through a genetic screen of the cancer translatome.

Cell reports

Kuzuoglu-Ozturk D, Hu Z, Rama M, Devericks E, Weiss J, Chiang GG, Worland ST, Brenner SE, Goodarzi H, Gilbert LA, Ruggero D

Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing.

Cell

Nuñez JK, Chen J, Pommier GC, Cogan JZ, Replogle JM, Adriaens C, Ramadoss GN, Shi Q, Hung KL, Samelson AJ, Pogson AN, Kim JYS, Chung A, Leonetti MD, Chang HY, Kampmann M, Bernstein BE, Hovestadt V, Gilbert LA, Weissman JS

Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression.

Cell

Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA

Mapping cancer genetics at single-cell resolution.

Science translational medicine

Gilbert LA

The DNA methylation landscape of advanced prostate cancer.

Nature genetics

Zhao SG, Chen WS, Li H, Foye A, Zhang M, Sjöström M, Aggarwal R, Playdle D, Liao A, Alumkal JJ, Das R, Chou J, Hua JT, Barnard TJ, Bailey AM, Chow ED, Perry MD, Dang HX, Yang R, Moussavi-Baygi R, Zhang L, Alshalalfa M, Laura Chang S, Houlahan KE, Shiah YJ, Beer TM, Thomas G, Chi KN, Gleave M, Zoubeidi A, Reiter RE, Rettig MB, Witte O, Yvonne Kim M, Fong L, Spratt DE, Morgan TM, Bose R, Huang FW, Li H, Chesner L, Shenoy T, Goodarzi H, Asangani IA, Sandhu S, Lang JM, Mahajan NP, Lara PN, Evans CP, Febbo P, Batzoglou S, Knudsen KE, He HH, Huang J, Zwart W, Costello JF, Luo J, Tomlins SA, Wyatt AW, Dehm SM, Ashworth A, Gilbert LA, Boutros PC, Farh K, Chinnaiyan AM, Maher CA, Small EJ, Quigley DA, Feng FY

Pharmaceutical-Grade Rigosertib Is a Microtubule-Destabilizing Agent.

Molecular cell

Jost M, Chen Y, Gilbert LA, Horlbeck MA, Krenning L, Menchon G, Rai A, Cho MY, Stern JJ, Prota AE, Kampmann M, Akhmanova A, Steinmetz MO, Tanenbaum ME, Weissman JS

Keap1ing an eye on Slc33A1.

Nature Cancer

Gilbert LA

Clonal ZEB1-driven mesenchymal transition promotes targetable oncologic anti-angiogenic therapy resistance.

Cancer research

Chandra A, Jahangiri A, Chen W, Nguyen AT, Yagnik G, Pereira MP, Jain S, Garcia JH, Shah SS, Wadhwa H, Joshi RS, Weiss J, Wolf KJ, Lin JG, Müller S, Rick JW, Diaz AA, Gilbert LA, Kumar S, Aghi MK

Exploring genetic interaction manifolds constructed from rich single-cell phenotypes.

Science (New York, N.Y.)

Norman TM, Horlbeck MA, Replogle JM, Ge AY, Xu A, Jost M, Gilbert LA, Weissman JS

A Bounty of New Challenging Targets in Oncology for Chemical Discovery.

Biochemistry

Lou K, Gilbert LA, Shokat KM

DNA-dependent protein kinase drives prostate cancer progression through transcriptional regulation of the Wnt signaling pathway.

Clinical cancer research : an official journal of the American Association for Cancer Research

Kothari V, Goodwin JF, Zhao SG, Drake JM, Yin Y, Chang SL, Evans JR, Wilder-Romans K, Gabbara K, Dylgjeri E, Chou J, Sun G, Tomlins SA, Mehra R, Hege K, Filvaroff EH, Schaeffer EM, Karnes RJ, Quigley DA, Rathkopf D, He HH, Speers C, Spratt DE, Gilbert LA, Ashworth A, Chinnaiyan AM, Raj GV, Knudsen KE, Feng FY

KRASG12C inhibition produces a driver-limited state revealing collateral dependencies.

Science signaling

Lou K, Steri V, Ge AY, Hwang YC, Yogodzinski CH, Shkedi AR, Choi ALM, Mitchell DC, Swaney DL, Hann B, Gordan JD, Shokat KM, Gilbert LA

Lethal clues to cancer-cell vulnerability.

Nature

Feng FY, Gilbert LA

Cellular response to small molecules that selectively stall protein synthesis by the ribosome.

PLoS genetics

Liaud N, Horlbeck MA, Gilbert LA, Gjoni K, Weissman JS, Cate JHD

Functional Genomics for Cancer Research: Applications In Vivo and In Vitro.

Annual Review of Cancer Biology

Thomas A. O'Loughlin, Luke A. Gilbert

Finding and Interpreting Genetic Interactions using Perturb-Seq Single Cell RNA-Seq CRISPR Screens.

Biophysical Journal

Jonathan Weissman, Thomas Norman, Max Horlbeck, Luke Gilbert

DRES-01. ZEB1-MEDIATED INVASIVE MESENCHYMAL TRANSITION AT THE SINGLE CELL LEVEL PROMOTES ANTI-ANGIOGENIC THERAPY RESISTANCE IN GLIOBLASTOMA.

Neuro-Oncology

Ankush Chandra, Arman Jahangiri, William Chen, Garima Yagnik, Joseph Garcia, Alan Nguyen, Jacob Weiss, Kayla Wolf, Jung-Ming Lin, Soeren Mueller, Jonathan Rick, Aaron Diaz, Luke Gilbert, Sanjay Kumar, Manish Aghi

Genomic Hallmarks and Structural Variation in Metastatic Prostate Cancer.

Cell

Quigley DA, Dang HX, Zhao SG, Lloyd P, Aggarwal R, Alumkal JJ, Foye A, Kothari V, Perry MD, Bailey AM, Playdle D, Barnard TJ, Zhang L, Zhang J, Youngren JF, Cieslik MP, Parolia A, Beer TM, Thomas G, Chi KN, Gleave M, Lack NA, Zoubeidi A, Reiter RE, Rettig MB, Witte O, Ryan CJ, Fong L, Kim W, Friedlander T, Chou J, Li H, Das R, Li H, Moussavi-Baygi R, Goodarzi H, Gilbert LA, Lara PN, Evans CP, Goldstein TC, Stuart JM, Tomlins SA, Spratt DE, Cheetham RK, Cheng DT, Farh K, Gehring JS, Hakenberg J, Liao A, Febbo PG, Shon J, Sickler B, Batzoglou S, Knudsen KE, He HH, Huang J, Wyatt AW, Dehm SM, Ashworth A, Chinnaiyan AM, Maher CA, Small EJ, Feng FY

A high-throughput screen of real-time ATP levels in individual cells reveals mechanisms of energy failure.

PLoS biology

Mendelsohn BA, Bennett NK, Darch MA, Yu K, Nguyen MK, Pucciarelli D, Nelson M, Horlbeck MA, Gilbert LA, Hyun W, Kampmann M, Nakamura JL, Nakamura K

Genomic Hallmarks and Structural Variation in Metastatic Prostate Cancer.

Cell

Quigley DA, Dang HX, Zhao SG, Lloyd P, Aggarwal R, Alumkal JJ, Foye A, Kothari V, Perry MD, Bailey AM, Playdle D, Barnard TJ, Zhang L, Zhang J, Youngren JF, Cieslik MP, Parolia A, Beer TM, Thomas G, Chi KN, Gleave M, Lack NA, Zoubeidi A, Reiter RE, Rettig MB, Witte O, Ryan CJ, Fong L, Kim W, Friedlander T, Chou J, Li H, Das R, Li H, Moussavi-Baygi R, Goodarzi H, Gilbert LA, Lara PN, Evans CP, Goldstein TC, Stuart JM, Tomlins SA, Spratt DE, Cheetham RK, Cheng DT, Farh K, Gehring JS, Hakenberg J, Liao A, Febbo PG, Shon J, Sickler B, Batzoglou S, Knudsen KE, He HH, Huang J, Wyatt AW, Dehm SM, Ashworth A, Chinnaiyan AM, Maher CA, Small EJ, Feng FY

Mapping the Genetic Landscape of Human Cells.

Cell

Horlbeck MA, Xu A, Wang M, Bennett NK, Park CY, Bogdanoff D, Adamson B, Chow ED, Kampmann M, Peterson TR, Nakamura K, Fischbach MA, Weissman JS, Gilbert LA

Exploration of Benzothiazole-Rhodacyanines as Allosteric Inhibitors of Protein-Protein Interactions with Heat Shock Protein 70 (Hsp70).

Journal of medicinal chemistry

Shao H, Li X, Moses MA, Gilbert LA, Kalyanaraman C, Young ZT, Chernova M, Journey SN, Weissman JS, Hann B, Jacobson MP, Neckers LM, Gestwicki JE

Combinatorial genetics in liver repopulation and carcinogenesis with a novel in vivo CRISPR activation platform.

Hepatology (Baltimore, Md.)

Wangensteen KJ, Wang YJ, Dou Z, Wang AW, Mosleh-Shirazi E, Horlbeck MA, Gilbert LA, Weissman JS, Berger SL, Kaestner KH

The Promise and Challenge of In Vivo Delivery for Genome Therapeutics.

ACS chemical biology

Wilson RC, Gilbert LA

Combined CRISPRi/a-Based Chemical Genetic Screens Reveal that Rigosertib Is a Microtubule-Destabilizing Agent.

Molecular cell

Jost M, Chen Y, Gilbert LA, Horlbeck MA, Krenning L, Menchon G, Rai A, Cho MY, Stern JJ, Prota AE, Kampmann M, Akhmanova A, Steinmetz MO, Tanenbaum ME, Weissman JS

A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response.

Cell

Adamson B, Norman TM, Jost M, Cho MY, Nuñez JK, Chen Y, Villalta JE, Gilbert LA, Horlbeck MA, Hein MY, Pak RA, Gray AN, Gross CA, Dixit A, Parnas O, Regev A, Weissman JS

CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells.

Science (New York, N.Y.)

Liu SJ, Horlbeck MA, Cho SW, Birk HS, Malatesta M, He D, Attenello FJ, Villalta JE, Cho MY, Chen Y, Mandegar MA, Olvera MP, Gilbert LA, Conklin BR, Chang HY, Weissman JS, Lim DA

Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation.

eLife

Horlbeck MA, Gilbert LA, Villalta JE, Adamson B, Pak RA, Chen Y, Fields AP, Park CY, Corn JE, Kampmann M, Weissman JS

A senescence secretory switch mediated by PI3K/AKT/mTOR activation controls chemoprotective endothelial secretory responses.

Genes & development

Bent EH, Gilbert LA, Hemann MT

Ligand-binding domains of nuclear receptors facilitate tight control of split CRISPR activity.

Nature communications

Nguyen DP, Miyaoka Y, Gilbert LA, Mayerl SJ, Lee BH, Weissman JS, Conklin BR, Wells JA

Versatile in vivo regulation of tumor phenotypes by dCas9-mediated transcriptional perturbation.

Proceedings of the National Academy of Sciences of the United States of America

Braun CJ, Bruno PM, Horlbeck MA, Gilbert LA, Weissman JS, Hemann MT

Parallel shRNA and CRISPR-Cas9 screens enable antiviral drug target identification.

Nature chemical biology

Deans RM, Morgens DW, Ökesli A, Pillay S, Horlbeck MA, Kampmann M, Gilbert LA, Li A, Mateo R, Smith M, Glenn JS, Carette JE, Khosla C, Bassik MC

Versatile protein tagging in cells with split fluorescent protein.

Nature communications

Kamiyama D, Sekine S, Barsi-Rhyne B, Hu J, Chen B, Gilbert LA, Ishikawa H, Leonetti MD, Marshall WF, Weissman JS, Huang B

Nucleosomes impede Cas9 access to DNA in vivo and in vitro.

eLife

Horlbeck MA, Witkowsky LB, Guglielmi B, Replogle JM, Gilbert LA, Villalta JE, Torigoe SE, Tjian R, Weissman JS

CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs.

Cell stem cell

Mandegar MA, Huebsch N, Frolov EB, Shin E, Truong A, Olvera MP, Chan AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, Weissman JS, Qi LS, So PL, Conklin BR

Abstract IA27: Retooling CRISPR to turn genes on and off.

New Technologies in Cancer Research

Luke A. Gilbert, Max A. Horlbeck, Jacqueline Villalta, Shao Hao, Jason E. Gestwicki, Jonathan S. Weissman

Unraveling the Mechanism of SBRT Response in EGFR-Addicted Pulmonary Adenocarcinoma by CRISPR/Cas9 Functional Genomics.

International Journal of Radiation Oncology • Biology • Physics

A.L. Polishchuk, J. Flanagan, L.A. Gilbert, M. Horlbeck, X. Yang, A. Olow, M. Bassik, J.S. Weissman, D.A. Haas-Kogan, J.A. Wells, T.G. Bivona

Next-generation libraries for robust RNA interference-based genome-wide screens.

Proceedings of the National Academy of Sciences of the United States of America

Kampmann M, Horlbeck MA, Chen Y, Tsai JC, Bassik MC, Gilbert LA, Villalta JE, Kwon SC, Chang H, Kim VN, Weissman JS

Engineering complex synthetic transcriptional programs with CRISPR RNA scaffolds.

Cell

Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M, Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA

Abstract IA10: Improving existing cancer therapy by targeting the tumor microenvironment.

Target Discovery and Validation

Christian Pallasch, Luke Gilbert, Michael Hemann

A protein-tagging system for signal amplification in gene expression and fluorescence imaging.

Cell

Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD

Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation.

Cell

Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M, Weissman JS

Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System.

Cell

Baohui Chen, Luke A. Gilbert, Beth A. Cimini, Joerg Schnitzbauer, Wei Zhang, Gene-Wei Li, Jason Park, Elizabeth H. Blackburn, Jonathan S. Weissman, Lei S. Qi, Bo Huang

Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system.

Cell

Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS, Huang B

CRISPR interference (CRISPRi) for sequence-specific control of gene expression.

Nature protocols

Larson MH, Gilbert LA, Wang X, Lim WA, Weissman JS, Qi LS

Abstract PR07: Systematic genetic interaction maps reveal rewiring of the stress response network and resulting vulnerabilities in leukemia and multiple myeloma cells.

Interpreting the Cancer Genome

Martin Kampmann, Diego Acosta-Alvear, Min Cho, Yuwen Chen, Xiaokai Li, Luke Gilbert, Blake T. Aftab, Jason Gestwicki, Peter Walter, Jonathan S. Weissman

A thioredoxin family protein of the apicoplast periphery identifies abundant candidate transport vesicles in Toxoplasma gondii.

Eukaryotic cell

DeRocher AE, Coppens I, Karnataki A, Gilbert LA, Rome ME, Feagin JE, Bradley PJ, Parsons M

Toxoplasma gondii targets a protein phosphatase 2C to the nuclei of infected host cells.

Eukaryotic cell

Gilbert LA, Ravindran S, Turetzky JM, Boothroyd JC, Bradley PJ

You are here

Luke Gilbert, PhD